According to clinical statistics, about 10-15% of pregnant women have experienced miscarriage, the most would occur in the first trimester of pregnancy, and about 65% of miscarriages are caused by genetic factors. The chromosome abnormalities account for more than 86% of genetic factors, including Trisomy (60%), X monosomy (14%), and triploid (12%). Depend on the statistic report, about 5% of couples may suffer from recurrent miscarriage. The analysis of miscarriage biopsy can figure out whether the cause has resulted from chromosomal abnormalities of fetus or parents. It could provide genetic guidance for the next pregnancy, and assist doctors in choosing the medical treatments to reduce the incidence of repeated miscarriage and the physical and psychological harm to family, especially pregnant women.

Using fluorescent PCR-capillary electrophoresis to amplify multiplex PCR in asingle-tube reaction to quantitatively detect multiple Short Tandem Repeat(STR) polymorphism sites in 23 chromosomes with four-tube amplification. The results could assist in the diagnosis of the Trisomy, X monosomy, 69, XXY and 69, XXX as a reference for the treatment of recurrent or repeatedmiscarriage.